Non-radioactive dot-blot DNA reassociation for unequivocal yeast identification.

A rapid method of non radioactive Northern blot analysis.

The electrophoretic determination of RNA size by Northern blot analysis is an important procedure that has required to date the use of radiolabelled cDNA and cRNA probes. Here, we describe a new method which allows the size of an mRNA to be visualized by use of oligonucleotide probes directly labelled with alkaline phosphatase (AP). One advantage compared to radiolabelled probes is that AP-labe...

Measurement of telomere DNA content by dot blot analysis

Telomeres play a central role in human cancer, cardiovascular aging and possibly longevity. However, present methods to measure telomere length are fraught with shortcomings that limit their use. Here, we describe a novel method to measure the relative telomere DNA content by dot blot analysis. In each dot, the DNA content is measured by a DNA stain (Dx) and the telomeric DNA content is measure...

Reassociation kinetics of non-histone-bound DNA sites.

DNA-binding non-histone proteins (NHP) from rat liver have been prepared by utilizing their affinity toward phosphocellulose. The specifity of the DNA binding sites was investigated by the reassociation kinetics of the protein-bound DNA. Isolated protein-bound DNA (350 nucleotides) reassociated with a a-fold increase in the content of middle repetitive sequences as found in the whole rat genome...

Intrinsic catalase dot blot immunoassay for identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare.

The heat-labile T class of mycobacterial catalase exhibits peroxidase activity with some substrates. Most species of mycobacteria produce T-catalase, which is serologically characterized by a combination of shared epitopes and unique, species-specific epitopes. Antibodies to T-catalases from Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare have been cross absorb...

Non-radioactive Labeling of DNA and RNA